Disease gene edited in human embryos in scientific first; But method at centre of legal war

 02 Aug 2017 - 20:21

Disease gene edited in human embryos in scientific first; But method at centre of legal war
This sequence of images shows the development of embryos after being injected with biological kit to edit their DNA, removing a genetic mutation known to cause hypertrophic cardiomyopathy. (Oregon Health & Science University)


Paris:  Scientists in the United States have repaired a disease-causing mutation in the DNA of early-stage human embryos, they said Wednesday, taking a step closer to engineering babies free of inherited disorders.

The team's successful use of the CRISPR "gene editing" tool in viable embryos was hailed as a technical feat by outside experts, who called at the same time for deeper debate on the ethics of altering human DNA.

The lab-created, edited embryos were not allowed to develop beyond a few days, when they comprised a handful of cells.

Just over 72 percent of the embryos -- 42 out of 58 -- ended up being free of a heart disease-causing mutation carried in the DNA of the sperm used to create them, a team reported in the journal Nature.

This was an improvement on the 50-percent odds for embryos that result naturally from a couple in which one partner carries the coding error.

With further improvement, said study co-author Paula Amato of the Oregon Health & Science University, the method "can potentially be used to prevent transmission of genetic disease to future generations."

The alteration itself is heritable, meaning that the children and grandchildren of a person born with edited DNA will be safe from the same genetic disease.

Further research is needed to determine the technique's safety, and to boost its accuracy to as close to 100 percent as possible before it can be used to create embryos meant to develop into healthy babies.

"I am quite sure that there are tools that we could use to improve... this repair so that we could achieve say 90 to maybe 100-percent efficiency, then I would say we would be ready to move to clinical trials," said Amato's colleague Shoukhrat Mitalipov.

Gene editing is controversial because it evokes a future in which humans can order "designer" babies with specific features -- blonde hair, athleticism, perhaps even intelligence.

But there is also the prospect of avoiding heritable, genetic diseases that can handicap or kill.

Amato, Shoukhrat and a team studied a gene mutation which causes hypertrophic cardiomyopathy, a hereditary disease of the heart muscle which can translate into sudden heart failure and death, notably in athletes.

No longer fantasy

It is a type of disorder, like Huntington's Disease, that requires an abnormal gene from just one parent.

The researchers used sperm from a donor carrying the mutation, and eggs from healthy women.

They injected the CRISPR-Cas9 editing tool into the eggs at the same time as the sperm -- a major departure from what other teams have done.

The researchers found that 72.4 percent of the embryos, not 50 percent as would have been expected, were free of the errant gene.

Nor were there unintended mutations in other parts of the genome.

In previous CRISPR studies in China, the gene-editing tool was added only after fertilisation.

Those teams had problems with "mosaicism", which occurs when some cells in an embryo are corrected, and some not.

In the new study, just one embryo turned out a mosaic.

"We definitely want to replicate this study with other mutations and other donors," said Amato.

CRISPR is a revolutionary gene-editing technique which allows scientists to insert, remove and correct DNA within a cell with pinpoint precision.

For Peter Braude, a reproductive health expert from King's College London, the study showed that "germline genome editing has moved from future fantasy to the world of possibility."

The debate about using it in practice, he added, "needs to run to catch up."

"Perhaps the biggest question, and probably the one that will be debated the most, is whether we should be physically altering the genes of an IVF (lab-created) embryo at all," added Darren Griffin of the University of Kent, in Britain.

"Equally, the debate on how morally acceptable it is not to act when we have the technology to prevent these life-threatening diseases must also come into play."

Currently, the only way to avoid heritable disease in assisted reproduction, is to fertilise eggs in the lab, analyse the DNA of the resulting embryos, and eliminate those containing errors.

The new CRISPR technique could be used to increase the number of viable embryos for implantation, said the team, thus reducing the number of eggs that have to be invasively harvested.

In 2015, a UN bioethics committee called for a stop to human embryo gene editing for fears it could be used to modify the human race.

Last year, Britain granted scientists permission to edit embryo DNA in research on the causes of infertility and miscarriages.

And in February this year, a US science advisory committee said such modification should be allowed in future to eliminate disease.

"I am quite sure that there are tools that we could use to improve... this repair so that we could achieve say 90 to maybe 100-percent efficiency, then I would say we would be ready to move to clinical trials," says Shoukhrat Mitalipov.


As scientists announced Wednesday they had corrected a disease-causing gene mutation in human embryos for the first time, a legal dispute over who discovered the CRISPR-Cas9 "editing" tool they used, continues to rage.

With the potential to revolutionise medicine and agriculture, the fight over patent rights in the United States potentially involves vast sums of


Repeatedly mooted as a worthy candidate for the Nobel Prize in Chemistry, the technique allows scientists to remove and replace a faulty sequence on a strand of DNA with pinpoint precision.

The Cas9 protein is used as genetic "scissors" to eliminate the mutated gene, which is then replaced by a corrected version.

The method can theoretically be used to eliminate disease-bearing mutations in still-developing human embryos, or to engineer more nutritious and resilient plants and healthier livestock.


On the one hand, the technique is claimed by the French-American research duo of Emmanuel Charpentier, affiliated to the Max Planck Institute of Berlin, and Jennifer Doudna of the University of California, Berkeley.

They published a study in the prestigious journal Science in 2012, outlining how they used it to very precisely cut strands of isolated DNA.

In the other corner is Chinese-born American Feng Zhang of the Broad Institute in Massachusetts, who used CRISPR in 2013 to alter cells with a nucleus (eucaryotes) found in plants, animals and humans.


Berkeley filed for a patent with the US Patent and Trademark Office (USPTO) in 2012.

Zhang and Broad also applied, using a more costly fast-track procedure. Zhang claimed his application of the technique in eucaryotes was specific and distinct from the more general patent sought by the two women.

The case was challenged by Berkeley, but the US Patent and Trademark Office ruled in Zhang and Broad's favour, saying the work in eucaryotes had advanced on the foundation laid by Charpentier and Doudna.

Berkeley has lodged an appeal.

In the European Union and Britain, authorities have awarded CRISPR patents solely the Charpentier-Doudna duo.

And in June this year, the Chinese patent office similarly ruled that they qualified to seek sole copyright in that country.